Roles of TLR3 and RIG-I in Mediating the Inflammatory Response in Mouse Microglia following Japanese Encephalitis Virus Infection

Figure 7

Knockdown of RIG-I and TLR3 reduces the nuclear translocation of AP-1 and NF-κB. (a) BV-2 cells were transfected with RIG-I shRNA (RIG-I KD) or negative control shRNA (CTR) and then infected with JEV at an MOI of 1. AP-1 and NF-κB in the nucleus were detected with western blotting at 5 hpi. (d) BV-2 cells were transfected with TLR3 shRNA (TLR3 KD) or negative control shRNA (CTR) and then infected with JEV at an MOI of 1. AP-1 and NF-κB in the nucleus were detected with western blotting at 5 hpi. (b, c, e, f) The protein levels were quantified with immunoblot scanning and normalized to the amount of lamin A or β-tubulin. Error bars represent the standard deviation of results from three independent assays (^∗∗𝑃<0.01; ^∗∗∗𝑃<0.001).

(a)

(b)

(c)

(d)

(e)

(f)