Attenuation and Immunogenicity of a Live High Pathogenic PRRSV Vaccine Candidate with a 32-Amino Acid Deletion in the nsp2 Protein
Table 2
Nucleotide and amino acids changes among P5, 60, 80, and 100 of PRRSV strain GDQY1.
ORFs
Encoding protein (aa lengtha)
nt positionb
P5
P60
P80
P100
aa positionc
P5
P60
P80
P100
ORF1a
Nsp1 (180)
333
T
C
C
C
48
G
G
G
G
677
A
C
C
C
163
H
P
P
P
Nsp1 (203)
986
C
C
T
T
266
P
P
L
L
Nsp2 (950)
1586
A
G
A
G
466
D
G
D
G
1813
A
A
G
G
542
K
K
E
E
2000
A
A
A
G
604
Q
Q
Q
R
2018
T
T
T
G
610
I
I
I
T
2527
A
A
G
G
780
K
K
E
E
2560
A
A
A
G
791
M
M
M
V
2729–2830
—d
—
846–880
—
—
2986
A
G
G
G
933
E
G
G
G
3178
G
A
A
A
997
D
N
N
N
4220
G
G
A
A
1344
R
R
K
K
Nsp3 (446)
4447
C
C
C
T
1419
C
C
C
C
5120
C
C
C
T
1644
A
A
A
V
Nsp5 (170)
6076
A
G
G
G
1963
E
K
K
K
6156
C
T
T
T
1989
V
V
V
V
6157
A
C
C
C
1990
K
Q
Q
Q
6177
T
C
C
C
1996
F
F
F
F
6248
G
G
A
A
2053
L
L
L
L
6357
C
T
T
T
2056
A
A
A
A
Nsp7 (259)
7134
A
A
T
T
2315
K
K
N
N
ORF1b
Nsp9 (643)
8139
G
G
G
T
178
A
A
A
S
8310
G
G
A
A
235
E
E
K
K
8322
T
T
A
A
239
L
L
I
I
8427
C
T
T
T
274
P
S
S
S
8269
A
A
G
G
554
I
I
M
M
Nsp10 (441)
10895
C
C
T
T
408
F
F
F
F
Nsp11 (223)
11321
G
G
G
A
109
K
K
K
K
OR2a
GP2 (257)
12012
G
A
A
A
10
L
L
L
L
12131
A
A
T
T
50
Y
Y
F
F
12334
A
A
G
G
118
I
I
V
V
12377
G
G
A
A
132
S
S
N
N
12592
C
C
A
A
204
H
H
N
N
ORF2b
E (73)
12012
G
A
A
A
9
D
N
N
N
12131
A
A
T
T
48
L
L
F
F
ORF3
GP3 (255)
12840
C
T
T
T
79
H
Y
Y
Y
13032
T
C
C
C
143
F
L
L
L
13275
T
T
T
C
223
H
H
H
H
13277
G
G
A
A
224
Q
Q
Q
Q
ORF4
GP4 (179)
13275
T
T
T
C
42
S
S
S
P
13277
G
G
A
A
43
D
D
N
N
13561
C
C
T
T
137
H
H
H
H
ORF5
GP5 (200)
13853
G
G
G
T
52
G
G
G
V
13864
T
T
T
C
56
L
L
L
P
13936
G
T
G
T
80
G
V
G
V
14033
C
T
T
T
112
Y
Y
Y
Y
14284
A
G
G
G
196
Q
R
R
R
Amino acids length of each NSP.
bNucleotide position mapped in the viral genome.
cAmino acids position mapped in each nonstructural or structural protein.
dDash stands for deletions in those positions.
