Autoantibody Response to Murine Double Minute 2 Protein in Immunodiagnosis of Hepatocellular Carcinoma

Figure 2

Western blot analysis with representative sera in ELISA. Lane 1, the polyclonal anti-MDM2 antibody was used as positive control. Lanes 2–5, four representative HCC sera which were positive in ELISA also had strong reactivity with MDM2 recombinant protein in Western blot analysis. Lanes 6–8, three randomly selected NHS had negative reactivity with MDM2 recombinant protein.