Autoantibody Response to Murine Double Minute 2 Protein in Immunodiagnosis of Hepatocellular Carcinoma
Figure 4
Representative immunofluorescence staining pattern of anti-MDM2 autoantibody positive HCC serum (performed on Hep2 antinuclear antigen tissue slides). (a) NHS were used as negative control; (b) polyclonal anti-MDM2 antibody which showed a nuclear immunofluorescence staining pattern was used as positive control; (c) a representative anti-MDM2 autoantibody positive HCC serum demonstrated an intense nuclear staining pattern; (d) the same HCC serum used in panel (c) was preabsorbed with recombinant MDM2. The nuclear fluorescent staining was significantly reduced.
