Immunomodulation by Trypanosoma cruzi: Toward Understanding the Association of Dendritic Cells with Infecting TcI and TcII Populations

Figure 5

T. cruzi strains differentially affect the expression of the chemokine receptors CCR5 and CCR7 in DCs. Murine bone marrow-derived DCs were incubated for 18 h with no stimulation (medium), LPS (100 ng/mL), or different T. cruzi strains (MOI 3 : 1), and CCR7 or CCR5 expression was evaluated by flow cytometry. ((a) and (c)) Percentage of DCs expressing CCR5 or CCR7. ((b) and (d)) Intensity of CCR5 or CCR7 expression in DCs based on the MFI. The bars represent the mean, and the vertical lines represent the standard error. compared with noninfected DCs (medium). # and lines: comparing the strains; Student’s -test or Mann-Whitney test.

(a)

(b)

(c)

(d)