Expression of Neural Markers by Undifferentiated Mesenchymal-Like Stem Cells from Different Sources
Figure 9
Stem cells expression of βIII-tubulin and NeuN by immunoblotting. (a) Total protein extracts from hASC (1), hS-MSC (2), hPDLSC (3), hDPSC (4) and dorsal root ganglia (DRG) (5) cultures were separated by 13% SDS-PAGE and blotted with anti-βIII-tubulin antibody. A 50 kDa molecular weight band was evident in the protein extracts from all the stem cells of different sources examined, as in positive control (DRG). Actin was used as loading control. (b). Nuclear protein extracts from hASC (1), hS-MSC (2), hPDLSC (3), hDPSC (4), and DRG (5) cultures were separated by 13% SDS-PAGE and blotted with anti-NeuN antibody. All the different types of stem cells examined express the two major NeuN species at 45–50 kDa, a reactive band at ~66 kDa, and another one between 70–90 kDa, with an expression profile similar to control. Actin was used as loading control.
