fMLP-Induced IL-8 Release Is Dependent on NADPH Oxidase in Human Neutrophils

Figure 4

Nox2 siRNA interferes with ROS and IL-8 production in HL-60-derived neutrophilic cells. HL-60 cells were differentiated to neutrophils and transfected with Nox2 siRNA or control siRNA. (a) A representative immunoblot of Nox2 from cells untreated with siRNA or treated with unspecific siRNA (siControl) or specific siRNA (siNox2) is shown. As control β-actin was used. (b) HL-60/neutrophils transfected with siRNA siControl or Nox2 were loaded with HE and treated with vehicle (Control) or fMLP. The superoxide production was measured by flow cytometry. (c) HL-60/neutrophils untreated or treated with siRNA siControl or siNox2 were incubated with vehicle or fMLP for 4 h and IL-8 production in the supernatants by ELISA was analysed. Mean ± SE, compared to the siControl cells treated with fMLP, .

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