Intradermal DNA Electroporation Induces Cellular and Humoral Immune Response and Confers Protection against HER2/neu Tumor

Figure 3

Intradermal EP vaccination transfects dendritic cells that migrate to draining lymph nodes. Transfected (red) muscle fibers and cells (arrows) in skin. Nuclei are shown in green. Magnification ×400. (a) Transfected dendritic cells have migrated into lymph nodes (CD11c in green, EGFP in red). (b) Interaction of dendritic cells that have incorporated EGFP (in red) with CD4 cells (in green) (c). Transfected cells (in red) adjacent to B220 cells (in green) in draining lymph node. (d) Nuclei are shown in blue, magnification ×630. ((b)–(d)) Images are representative of three independent experiments.

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