Intradermal DNA Electroporation Induces Cellular and Humoral Immune Response and Confers Protection against HER2/neu Tumor

Figure 5

Intradermal EP vaccination induces cellular response in Her2/neu nontolerant mice and humoral immune response in both tolerant and nontolerant mice. In vivo cytotoxicity against the H- dominant peptide in BALB/c and BALB-neuT (each experimental group ) mice vaccinated with pVAX (gray bars) or pVAX-ECTM plasmid (black bars). Data are reported as % of lysis. ,, Student’s -test. (a) Anti-Her2/neu antibody titer (MFI ± SEM) in BALB/c (b) and BALB-neuT mice (c). ,, ,, Student’s -test ((b), (c)). Isotype titration, performed in three different pools of sera, revealed similar composition of Her2/neu specific antibodies for BALB/c (d) and BALB-neuT (e) treated mice ((d), (e)).

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