Intradermal DNA Electroporation Induces Cellular and Humoral Immune Response and Confers Protection against HER2/neu Tumor

Table 1

Semiquantitative analysis of inflammatory cells present in the skin of BALB/c mice from 3 to 96 hours after pVAX-EGFP vaccination followed by electroporation.

Time after intradermal EP vaccination	Gr-1	CD11b	CD68	CD11c	CD4	B220	CD8
3 h	+++	+++	++	+	+
6 h	++	+++	++	++	+++	+
24 h	++	++	+	+	++	+
48 h	+	++	+		+	++
72 h	+	++	+		+	++
96 h		++	+			++

The expression of reactive cells was defined as absent (−), scarcely (), moderately (+), frequently (++), or strongly (+++) present on cryostat sections stained with the antibodies.