|
| | Marker1 | Localization | Expressed by |
|
| Involved in establishing Tregs phenotype | FoxP3 | Nucleus | tTregs, pTregs (but not in some subsets), and CD8+ Tregs |
| Helios | Nucleus | tTregs, pTregs (occasionally) |
|
| Involved in Tregs regulatory activity and markers of active Tregs | IL-2 receptor (CD25) | Membrane (the level of expression is crucial) | tTregs, pTregs (but not in some subsets), and CD8+ Tregs |
| CTLA-4 | Membrane (but detectable only intracellularly) | tTregs, pTregs |
| TGF-β | Cytoplasm, secreted | pTregs (especially but not exclusively Th3) |
| IL-10 | Cytoplasm, secreted | pTregs (especially Tr1) |
| Granzyme B | Cytoplasm, secreted | tTregs, pTregs |
| Perforin | Cytoplasm, secreted | tTregs |
| IL-35 | Cytoplasm, secreted | pTregs (occasionally) |
| E5NT (CD73) | Membrane | pTregs |
| ENTPD1 (CD39) | Membrane | pTregs |
| PD-1 ligand (PD-L1) | Membrane | pTregs |
|
| Other markers of active Tregs | GITR (CD357) | Membrane | tTregs, pTregs, and CD8+ Tregs |
| T-cell Ig and mucin domain protein-3 (Tim-3) | Membrane | tTregs, pTregs |
| Galectin-9 | Membrane | tTregs, pTregs |
| ICOS (CD278) | Membrane | pTregs |
| latency-associated peptide (LAP) | Membrane | tTregs |
| CD69 (C-type lectin receptor) | Membrane | pTregs |
|
| Involved in tTregs differentiation | IL-2 receptor (CD25) | Membrane | tTregs |
| GITR (CD357) | Membrane | tTregs |
| OX40 (CD134) | Membrane | tTregs |
| TNFR2 | Membrane | tTregs |
|
| Involved in pTregs differentiation/expansion and tTregs expansion | IL-2 receptor (CD25) | Membrane | tTregs, pTregs (but not in some subsets) |
| CD28 | Membrane | tTregs, pTregs |
| GITR (CD357) | Membrane | tTregs, pTregs, and CD8+ Tregs |
| OX40 (CD134) | Membrane | tTregs, pTregs |
| ICOS (CD278) | Membrane | pTregs |
| LAG-3 (CD223) | Membrane | tTregs, pTregs |
| Programmed cell death (PD)-1 | Membrane | pTregs |
| PD-1 ligand (PD-L1) | Membrane | pTregs |
| CD226 | Membrane | pTregs (Tr1) |
| CD69 (C-type lectin receptor) | Membrane | pTregs |
|
| Involved in inhibition of Tregs activity | GITR (CD357) | Membrane | tTregs, pTregs, and CD8+ Tregs |
| OX40 (CD134) | Membrane | tTregs, pTregs |
| 4-1BB (CD137) | Membrane | tTregs, pTregs |
|
| Other markers | CD45R0 (memory marker) | Membrane | tTregs (those expanded), pTregs |
| CD45RA (naïve marker) | Membrane | tTregs |
| Neuropilin-1 (VEGF receptor) | Membrane | tTregs, low expression in pTregs |
| CD49b | Membrane | pTregs (Tr1) |
|
| Not expressed by Tregs | IL-7 receptor (CD127) | Membrane | tTregs |
| CD49d | Membrane | tTregs |
|
