A Novel PET Imaging Using ⁶⁴Cu-Labeled Monoclonal Antibody against Mesothelin Commonly Expressed on Cancer Cells

Figure 4

Binding activity of DOTA-conjugated 11-25 mAb and ⁶⁴Cu-DOTA-11-25 mAb to recombinant MSLN determined by ELISA as compared with native 11-25 mAb. Native 11-25 mAb (open triangles) and DOTA-conjugated 11-25 mAb (open squares) and ⁶⁴Cu-DOTA-11-25 mAb (closed diamonds) were added to the wells on which MSLN had been immobilized. Antibody binding was detected with peroxidase-labeled anti-mouse IgG and TMB. The reaction was stopped with 2 N H₂SO₄ and the absorbance at 450 nm was measured.