A Novel PET Imaging Using ⁶⁴Cu-Labeled Monoclonal Antibody against Mesothelin Commonly Expressed on Cancer Cells

Figure 6

Cell binding assay with ⁶⁴Cu-DOTA-11-25 mAb. BxPC-3 cells were transferred to 24-well plates at 5 × 10⁴ cells/well/mL and cultured for 4 days. Various concentrations of ⁶⁴Cu-DOTA-11-25 mAb were incubated with the cell monolayers for 2 hours on ice in complete growth media (RPMI-1640 medium containing 10% fetal bovine serum). The cells were washed and lysed and their radioactivity was counted in a γ-counter. Inner graph showed the Scatchard plot of the specific binding versus the concentration of ⁶⁴Cu-DOTA-11-25 mAb.