A Novel PET Imaging Using ⁶⁴Cu-Labeled Monoclonal Antibody against Mesothelin Commonly Expressed on Cancer Cells

Table 1

Concentration of soluble MSLN in the culture media of cancer cells.

Tissue	Disease	Cancer cell line	Soluble MSLN (ng/mL)
Pancreas	Ductal adenocarcinoma	CFPAC-1	57.9
Pancreas	Adenocarcinoma	BxPC-3	72.6
Pancreas	Epithelioid carcinoma	PANC-1	0.0
Lung	Biphasic mesothelioma	MSTO-211H	71.6
Lung	Mesothelioma	NCI-H226	23.7
Lung	Squamous cell carcinoma	NCI-H520	0.0

The cells were cultured for 5 days and the culture media were collected and centrifuged. The supernatant was subjected to a sandwich ELISA for soluble MSLN, as described in Section 2.