Development of Nonaggregating Poly-A Tailed Immunostimulatory A/D Type CpG Oligodeoxynucleotides Applicable for Clinical Use

Figure 10

D35 modification with dAs40 at 5′ and/or 3′ ends and their SPGylation effect on cytokine production from human PBMC. Human PBMCs were stimulated with ODNs (D35-dAs40, dAs40-D35, and D35-dAs40-D35; 1 M each) or their SPGylated ODNs (D35-SPG, SPG-D35, and D35-SPG-D35; 1 M each ODN amount), and then 24 hours later, IFN- and IL-6 secretion in supernatants were determined by ELISA. Bar graph shows mean ± SEM in triplicate. , , and .