Development of Nonaggregating Poly-A Tailed Immunostimulatory A/D Type CpG Oligodeoxynucleotides Applicable for Clinical Use

Figure 11

5′ addition of dAs40 sequence to D35 also prevents visible large aggregate formation in PBS. The indicated ODNs were initially dissolved in distilled water at a concentration of 10 mg/mL (all ODNs were completely solubilized with water and the solutions were clear) and further diluted with PBS at a final concentration of 1.0 mg/mL. Solutions were stored at 4°C for at least 18 hours and then images were captured. D35 developed visible white turbidity during this incubation. In contrast, dAs40-D35 did not develop visible white turbidity.