Development of Nonaggregating Poly-A Tailed Immunostimulatory A/D Type CpG Oligodeoxynucleotides Applicable for Clinical Use

Figure 4

Biological activities of D35-dAs40 and D35core-dAs40. (a) Dose-dependent IFN-α production from human PBMC by the indicated ODNs. (b) Effect of the adjacent sequence of cytokine inducing activity. D35core plus dAs40 is sufficient to induce IFN-α secretion from human PBMC. Bar graph indicates mean ± SEM in triplicate. (c) dAs tail length affects the biological activity of D35core-dAs type ODNs. Human PBMC stimulated with the indicated ODNs (final concentration = 1 μM), and after 24 h cytokine concentration was determined by ELISA. Bar graph indicates mean ± SEM in triplicate. (d) The tail length and ODN dose relations of the indicated ODNs. The bar graph shows dose-dependent IFN-α and IL-6 production from a single well by each stimulation. ; .

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