Development of Nonaggregating Poly-A Tailed Immunostimulatory A/D Type CpG Oligodeoxynucleotides Applicable for Clinical Use

Figure 9

3′ addition of dAs40 sequence to A2216 and A2336 improves direct solubilization in saline. (a) Lyophilized A2216 vial (10 mg/vial) (upper left) was completely solubilized with 1 mL of saline directly at room temperature (RT) (lower left). The solution turned to a gel at 4°C (upper right) and reliquefied at 37°C (lower right). (b) Lyophilized A2336 vial (10 mg/vial) (upper) formed many visible gelatinous aggregation with 1 mL of saline at RT (lower). (c) Lyophilized A2216-dAs40 (0.5 mg/vial) and A2336-dAs40 (0.5 mg/vial) were easily and completely dissolved with 50 μL of saline at RT. (d) Human PBMCs were stimulated with the indicated synthetic ODNs (1 μM) for 24 hours. IFN-α and IL-6 production in the supernatants were examined by ELISA. Bar indicates the mean SEM.

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