Differential Immune Response against Recombinant Leishmania donovani Peroxidoxin 1 and Peroxidoxin 2 Proteins in BALB/c Mice

Figure 3

Anti-LdPxn1 and -LdPxn2 antibodies in immunized mice. Mice were immunized subcutaneously with recombinant LdPxn1 or LdPxn2 proteins with or without CpG ODN or GLA-SE. Mice were boosted twice in 3-week intervals. The levels of IgG1 and IgG2a isotypes were measured on sera collected at different time points using ELISA. Data are presented as the mean OD S.E.M. of IgG1 and IgG2a of sera from mice immunized with rLdPxn1 (a) and rLdPxn2 (b). The IgG2a/IgG1 ratios are shown in tables below each figure.

(a)

(b)