The Novel PKCθ from Benchtop to Clinic

Figure 1

A schematic model of membrane-associated PKCθ in TCR/CD28 stimulated T cells. Regulatory N-terminal domain consists of V1, C2-like, V2, C1a, C1b, and V3 domains. Catalytic C-terminal domain consists of ATP binding site, V4, kinase catalytic domain (substrate binding site), and V5. PKCθ binds to the membrane through diacylglycerol by its C1a and C1b domains. It interacts with CD28 via Lck through its V3 domain. Blue arrows represent phosphorylation by respective enzymes on specific amino acid residues. V: variable domain; C: constant domain; GLK: germinal center kinase- (GSK-) like kinase; Lck: Lymphoid cell kinase; TCR: T cell receptor.