Dendritic Cell Activity Driven by Recombinant Mycobacterium bovis BCG Producing Human IL-18, in Healthy BCG Vaccinated Adults

Figure 2

Immunoblot analysis of rBCGhIL-18. Lane 1 contains 100 ng of mature IL-18. Whole-cell extracts of nonrecombinant BCG (lane 2) or rBCGhIL-18 (lane 3), each corresponding to an optical density at 600 nm of 0.2 were subjected to SDS-PAGE and analyzed by immunoblotting using rabbit anti-IL-18 antibodies. The sizes of the molecular weight markers are indicated in the left margin, and the presence of mature IL-18 and pro-IL-18 is indicated by arrows.