Vaccination with an Attenuated Ferritin Mutant Protects Mice against Virulent Mycobacterium tuberculosis

Figure 3

Genome-wide gene expression profiling of vaccinated and Mtb-infected mice lungs. (a) Principal component analysis of lung transcriptome data from bfrB (blue) or BCG (red) vaccinated, Mtb-infected mice. The eclipse around each group denotes the standard deviation of the datasets. (b) Volcano plot of lung global gene expression showing the value significance (-axis; log scale) and fold change (-axis). Upregulated genes are shaded in purple and downregulated genes are in yellow. N/C denotes no change. Each spot (blue) in the plot corresponds to a gene. (c) Intensity plot and dendrogram of significantly differentially expressed genes in the bfrB vaccinated, compared to BCG vaccinated, Mtb-infected mice lungs. Upregulated genes are in red and downregulated genes are in blue. The color scale bar ranges from +3 (red) to −3 (blue).

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