NKG2D Signaling Leads to NK Cell Mediated Lysis of Childhood AML

Figure 2

(a) Quantitative HLA I expression measured by Qifikit (Dako) was correlated to the mean NK cell mediated lysis (2 donors) of primary childhood AML blasts at E : T ratio 10 : 1. NK cells were obtained from two healthy volunteer donors. PBMCs were isolated from peripheral whole blood by density gradient centrifugation using Biocoll separating solution (Biochrom) followed by a two-step immunomagnetic CD56⁺ enrichment and subsequent CD3⁺ depletion. No correlation was found without IL2 prestimulation (; Pearson , , ). (b) Distribution of blast HLA type was C1/C1 (), C1/C2 (), and C2/C2 (). KIR ligand-ligand match versus mismatch did not reveal any difference in specific lysis of primary childhood AML blasts (, ;  ). Donor A revealed a KIR ligand-ligand match versus mismatch proportion (3 to 11) and donor B revealed a KIR ligand-ligand match versus mismatch proportion (7 to 7). (c) No correlation was found with IL2 prestimulation at E : T ratio 10 : 1 (; Pearson , , ). (d) There was no impact of KIR ligand-ligand match or mismatch on IL2 prestimulated NK cell mediated cytolysis of AML blasts.

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