Carbohydrate Microarrays Identify Blood Group Precursor Cryptic Epitopes as Potential Immunological Targets of Breast Cancer

Figure 1

Carbohydrate microarray analysis of antiepiglycanin mAb HAE3. Seventy-six glycoproteins, glycoconjugates, and polysaccharides were spotted in triplicates in 1 to 2 dilutions to yield the customized microarrays for antibody screening. (a) Microarray detections were shown as the mean fluorescent intensities (MFIs) of each microspot with antigen-binding signal in red and background reading in blue. Each error bar is constructed using one standard deviation from the mean of triplicate detections. The labeled antigens include HCA (ID# 1 and ID# 2), a number of blood group precursors (29^#–32^#), and a microarray spotting marker (80^#). (b) Images of a microarray stained with HAE3 (5 μg/mL). (c) Schematic of a blood group substance structure with the conserved O-glycan core highlighted.

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