NK and NKT Cell Depletion Alters the Outcome of Experimental Pneumococcal Pneumonia: Relationship with Regulation of Interferon-γ Production

Figure 1

Kaplan-Meier survival plots. (a) Mice were intratracheally challenged with Streptococcus pneumoniae after pretreatment with nonspecific IgG (CON, ); after pretreatment with anti-asialo GM1 antibody (NKd, ); or after pretreatment with anti-CD1d antibody (NKTd, ). Survival was recorded for 7 days after bacterial challenge. Ten sham-operated mice (Sham) were also followed up and none died. Comparisons log-: 10.19, : 0.001; log-: 1.96, : 0.161; and log-: 16.62; . (b) CD3−/NK1.1+ cell populations among splenocytes from each group. Results come from per group at the time of sacrifice, 48 hours after bacterial challenge. of comparisons with sham-operated mice: ; . (c) Data represent fluorescence-activated cell sorting plots obtained 48 hours after bacterial inoculation. Splenocytes were stained with fluorochrome labeled anti-NK1.1 and anti-CD3 monoclonal antibodies. CD3−/NK1.1+ (NK) cell populations in (A) control and (B) anti-asialo GM1 treated animals are depicted.

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