Polyclonal Recipient nTregs Are Superior to Donor or Third-Party Tregs in the Induction of Transplantation Tolerance

Figure 2

Therapeutic nTreg treatment induces multilineage chimerism at doses of 3, 1, and 0.5 × 10⁶ cells without cytoreduction. B6 BMT recipients were treated with different doses of recipient nTregs (■ 3 × 10⁶ cells, ; ◆ 1 × 10⁶ cells, ; ∆ 0.5 × 10⁶ cells, ; ○ 0.1 × 10⁶ cells, ). Donor (H-) chimerism among leukocytes of T cell (CD4⁺ and CD8⁺), B cell (B220⁺), and the myeloid (Mac1⁺) lineage was assessed by flow cytometry of peripheral blood at multiple time points and is shown as mean percent (error bars indicate standard deviation). Data are representative for at least 3 independent experiments per group.