Acceleration of Wound Healing by -gal Nanoparticles Interacting with the Natural Anti-Gal Antibody

Figure 5

Prevention of scar formation in GT-KO mouse wounds treated with α-gal nanoparticles. Representative wound treated for 28 days with α-gal nanoparticles and stained with H&E (a) and with trichrome (c). Representative saline treated wound after 28 days, stained with H&E (b) and with trichrome (d). Collagen is stained blue by trichrome. α-gal nanoparticles treated wounds (a, c) display restoration of normal skin structure, including thin epidermis, loose connective tissue in the dermis and appearance of skin appendages including hair, sebaceous glands, fat and muscle cells in the hypodermis. Saline treated wounds (b, d) undergo fibrosis to form a scar characterized by dense connective tissue as a result of extensive collagen secretion by multiple infiltrating fibroblasts, no skin appendages and hyperplastic epidermis. The hair shafts in the left are of the uninjured area bordering the wound. Scale bar in (d) is 100 μm (×100). Specimens are representative of 5 mice per group. Modified from [19].

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