Regulation of Murine Ovarian Epithelial Carcinoma by Vaccination against the Cytoplasmic Domain of Anti-Müllerian Hormone Receptor II

Figure 2

Immunogenicity of AMHR2-CD. Female C57BL/6 mice were immunized with AMHR2-CD in CFA, and LNC or splenocytes were cultured in vitro for assessment of proliferation and cytokine production. (a) Ten-day primed LNC showed marked antigen-specific recall proliferative responses to AMHR2-CD over several logs of antigen concentration. (b) The response to AMHR2-CD was elicited by CD4+ T cells but not by CD8+ T cells purified by magnetic bead separation. (c) Proliferative responses to AMHR2-CD were markedly inhibited in the presence of CD4 antibody but not in the presence of CD8 or isotype control antibodies. (d) Four weeks after immunization, splenocytes were reactivated with immunogen and ELISA analysis of 72-hour culture supernatants showed that recall responses to AMHR2-CD involved a proinflammatory phenotype with elevated production of IFNγ and minimal production of IL-2, IL-4, and IL-5. (e) Splenocyte production of IFNγ was elicited from purified CD4+ T cells but not from purified CD8+ T cells. (f) Two months after immunization, serum levels of AMHR2-CD-specific IgG were detectable even at titers over 1 : 50,000 dilution. PBS was substituted for diluted sera in the PBS control. Error bars show ±SD.

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