Decreased Frequency of Circulating Myelin Oligodendrocyte Glycoprotein B Lymphocytes in Patients with Relapsing-Remitting Multiple Sclerosis

Figure 2

Reactivity of MOG-specific B cells. B cells were preincubated with 20 μg of soluble MOG, albumin, dsDNA, insulin, or LPS before the addition of MOG-coated beads (). (a) The frequency of MOG-BBR drops with the soluble MOG preincubation but not with albumin preincubation. (b) The recognition of MOG-coated beads decreased after the addition of soluble LPS (% of inhibition: 73%), dsDNA (11%), and insulin (35%). Competitive assay was tested in MS (); the percentage of inhibition after soluble antigen addition was the same proportion as HI: LPS (78%), dsDNA (11%), and insulin (32%). (c) B cells were preincubated with Fab’2 anti-human IgG+IgA+IgM. The frequency of B cells recognizing MOG-coated beads was assessed on 3 HI. Fab’2 antibody modified the frequency of MOG-specific B cells (ns, , Wilcoxon test).

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