Decreased Frequency of Circulating Myelin Oligodendrocyte Glycoprotein B Lymphocytes in Patients with Relapsing-Remitting Multiple Sclerosis

Figure 6

Intrathecal MOG-specific B cells in CIS and patients with non-MS-related pathologies. (a) The gating strategy of CSF analysis is represented. The number of B cells in this CIS sample was 1771 cells. The BBR frequency was obtained by FlowJo analysis which calculated the percentage of MOG-specific B cells among B cell subsets: 107 BBR corresponds to 5.69%. (b) Cells from fresh CSF samples were stained with CD19 antibody and incubated with rMOG and human albumin coated beads. After exclusion of dead cells (DAPI⁺), the frequency of BBR recognizing rMOG and albumin was assessed in CIS () and control patients () (ns, ). Mann-Whitney test and Wilcoxon test were used to compare albumin-BBR and MOG-BBR in CIS () and control patients () (c). The frequency of circulating and intrathecal MOG-BBR was assessed in CIS () and control patients (). Intrathecal frequency of MOG-BBR was higher than circulating frequency (ns, , Mann-Whitney test).

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