Renal and Hematological Effects of CLCF-1, a B-Cell-Stimulating Cytokine of the IL-6 Family

Figure 4

Immortalized podocytes were incubated with either (a) vehicle (b, c) 10 ng/mL CLCF-1 for (b) 15 minutes or (c) 30 minutes. Subsequently, cellular actin was stained as described in Methods and Materials and 0.25 µm sections were visualized by confocal microscopy. Note parallel actin bundles in central portion of control cell (a), compared to the decrease in intensity of central actin bundles and loss of parallel pattern in the cells treated with CLCF-1 (c). Lamellipodia are present in nearly the entire circumference after 30 minutes of incubation (c).

(a)

(b)

(c)