Experimental Immunization Based on Plasmodium Antigens Isolated by Antibody Affinity

Figure 6

Course of infection in BALB/c mice after antigen and Freund’s adjuvant vaccination. Groups of BALB/c mice were immunized with 10 μg of purified antigens formulated with Freund’s adjuvant (Ag + adjuvant, ), Freund’s adjuvant plus PBS (Adjuvant, ), or PBS alone (PBS, ). Two weeks after the fourth immunization, mice were challenged with 2 × 10⁷ PyL-RBCs. Percentages of (a) survival and (b) parasitemia from a total of 500 RBCs are shown as mean (± SEM). (c) Parasitemia course of mice with longer surviving rates after vaccination: mouse which survived around two weeks (Δ) or completely survived (). Data is shown as mean (± SEM). comparing group “Ag + adjuvant” to both controls.

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