EPIPOX: Immunoinformatic Characterization of the Shared T-Cell Epitome between Variola Virus and Related Pathogenic Orthopoxviruses

Figure 2

EPIPOX input page. The input page of EPIPOX is divided in two main sections for intuitive use. In the first part (SEARCH), users select HLA molecules and proteins to retrieve T-cell epitopes (multiple selection is allowed) while in the second part the user can limit the search output according to various criteria. These criteria include temporal expression of gene products (E: early; I: intermediate; L: late), location of proteins in relevant structures of the virus (CORE, IMV, and EEV), and the presence of leader and transmembrane regions. In addition, users can select only those peptides with a relative score above some selectable value. HLA-specific profiles used to score T-cell epitopes can reach a maximum score, which is used to set the relative score in percentage of each peptide. For HLA I-restricted epitopes, users can also restrict the search to those epitopes potentially generated by the proteasome.