Increased NY-ESO-1 Expression and Reduced Infiltrating CD3+ T Cells in Cutaneous Melanoma

Figure 1

Immunohistochemical detection of NY-ESO-1 (+) tumor cells and CD3+, CD8+ FOXP3−, CD8−FOXP3+, and CD8+FOXP3+ infiltrating lymphocytes in primary cutaneous melanoma. (a) NY-ESO-1 regional expression, 3+ intensity (×400); (b) NY-ESO-1 complete expression, 3+ intensity (×200); (c) CD3+ lymphocyte (red, arrow) embracing a melanoma cell (×630); (d) CD8+FOXP3− lymphocytes (membranous purple staining), CD8−FOXP3+ lymphocytes (blue nuclear staining, arrowhead), and CD8+FOXP3+ cell (purple membranous and blue nuclear staining, arrow), ×400.

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