DC-Based Immunotherapy Combined with Low-Dose Methotrexate Effective in the Treatment of Advanced CIA in Mice

Figure 6

SmDC inhibited Th1 response and induced Treg response regardless of MTX in coculture with arthritogenic T cells. Arthritogenic T cells, collected from CIA mice 21 days after CII-inoculation, were cocultured with smDC in the presence or absence of MTX up to 5000 nM. (a) CD4⁺IFN-γ⁺ Th1 cells were analyzed by FACS. (b) CD4⁺Foxp3⁺ Treg cells were analyzed by FACS. Statistical data are shown from 3 different mice as mean ± SEM. , in Student’s -test.

(a)

(b)