Aeromonas salmonicida Infection Only Moderately Regulates Expression of Factors Contributing to Toll-Like Receptor Signaling but Massively Activates the Cellular and Humoral Branches of Innate Immunity in Rainbow Trout (Oncorhynchus mykiss)
Table 3
(a) Expression profiles of various PRRs in selected tissues of rainbow trout at different time points after infection. (b) Expression profiles of various downstream factors in selected tissues of rainbow trout at different time points after infection.
(a)
Gene symbol
Tissues
Fold change values at time points after infection relative to control
6 h
12 h
24 h
48 h
72 h
tlr1
Spleen
−1.07 ± 0.19
−1.02 ± 0.23
1.07 ± 0.16
−1.07 ± 0.18
−1.54 ± 0.28
Head kidney
1.61 ± 0.18
1.72 ± 0.25
1.50 ± 0.27
1.57 ± 0.21
1.66 ± 0.13
Liver
−1.11 ± 0.18
1.72 ± 0.31
2.32 ± 0.56
1.67 ± 0.31
1.70 ± 0.22
Thymus
−1.31 ± 0.29
−1.20 ± 0.32
1.21 ± 0.29
1.13 ± 0.24
1.09 ± 0.20
tlr2
Spleen
−1.13 ± 0.16
−1.73 ± 0.33
−1.86 ± 0.41
−1.46 ± 0.15
−1.68 ± 0.20
Head kidney
1.24 ± 0.19
−1.13 ± 0.17
−1.68 ± 0.28
−1.10 ± 0.22
−1.18 ± 0.15
Liver
−1.01 ± 0.11
−1.11 ± 0.11
−1.01 ± 0.12
1.50 ± 0.32
1.16 ± 0.18
Thymus
−1.12 ± 0.21
−1.07 ± 0.19
−1.07 ± 0.25
1.16 ± 0.19
1.22 ± 0.16
tlr3
Spleen
−1.58 ± 0.17
−1.67 ± 0.25
−1.21 ± 0.25
−1.82 ± 0.23
−1.70 ± 0.23
Head kidney
−1.52 ± 0.17
−1.99 ± 0.26
−2.49 ± 0.30
−2.08 ± 0.29
−2.09 ± 0.32
Liver
−1.21 ± 0.31
−1.64 ± 0.39
−1.40 ± 0.36
−1.83 ± 0.47
−1.64 ± 0.51
Thymus
−1.26 ± 0.27
−1.87 ± 0.49
−1.46 ± 0.37
−1.52 ± 0.37
−1.63 ± 0.34
tlr5
Spleen
−2.77 ± 0.71
−3.88 ± 0.71
−2.76 ± 0.70
−2.34 ± 0.66
−1.61 ± 0.49
Head kidney
−1.07 ± 0.27
1.03 ± 0.64
−2.33 ± 0.48
−1.62 ± 0.47
−1.55 ± 0.34
Liver
1.28 ± 0.31
−1.30 ± 0.25
1.77 ± 0.37
1.99 ± 0.37
1.96 ± 0.38
Thymus
1.14 ± 0.23
−1.12 ± 0.29
−2.08 ± 0.35
−1.29 ± 0.30
−1.91 ± 0.52
tlr7
Spleen
−2.10 ± 0.24
−1.62 ± 0.58
−2.79 ± 0.48
−3.23 ± 0.62
−3.40 ± 0.85
Head kidney
1.95 ± 0.45
2.22 ± 0.63
1.51 ± 0.49
−1.28 ± 0.35
−1.04 ± 0.24
Liver
−1.01 ± 0.23
1.06 ± 0.19
1.17 ± 0.20
1.33 ± 0.41
−1.05 ± 0.30
Thymus
−1.30 ± 0.28
−1.34 ± 0.33
−1.03 ± 0.40
−1.35 ± 0.28
−1.47 ± 0.31
tlr8a1
Spleen
−2.03 ± 0.29
−1.76 ± 0.28
−1.29 ± 0.20
−1.36 ± 0.23
−1.62 ± 0.29
Head kidney
−1.35 ± 0.22
−1.89 ± 0.39
−1.90 ± 0.34
−1.30 ± 0.45
−1.89 ± 0.77
Liver
1.47 ± 0.41
1.81 ± 0.42
2.86 ± 0.77
2.88 ± 1.00
1.67 ± 0.43
Thymus
1.02 ± 0.21
−1.01 ± 0.27
−1.09 ± 0.28
1.15 ± 0.28
−1.41 ± 0.30
tlr8a2
Spleen
−2.45 ± 0.53
−1.99 ± 0.29
−1.49 ± 0.27
−1.85 ± 0.32
−2.75 ± 0.45
Head kidney
−1.31 ± 0.43
−1.46 ± 0.31
−1.23 ± 0.24
−1.29 ± 0.27
−1.91 ± 0.31
Liver
1.54 ± 0.50
1.75 ± 0.56
2.30 ± 0.56
2.56 ± 0.81
1.54 ± 0.42
Thymus
1.07 ± 0.27
−1.49 ± 0.33
−1.27 ± 0.32
1.03 ± 0.45
−1.28 ± 0.47
tlr9
Spleen
1.09 ± 0.11
1.32 ± 0.11
1.44 ± 0.17
1.20 ± 0.22
−2.00 ± 0.25
Head kidney
1.33 ± 0.19
1.35 ± 0.25
1.24 ± 0.21
1.29 ± 0.18
1.43 ± 0.17
Liver
1.50 ± 0.22
1.95 ± 0.32
2.57 ± 0.42
2.87 ± 0.52
3.44 ± 0.51
Thymus
−1.22 ± 0.37
−1.20 ± 0.40
1.15 ± 0.26
1.20 ± 0.39
1.33 ± 0.42
tlr19
Spleen
−1.22 ± 0.28
−1.66 ± 0.59
−1.62 ± 0.46
1.45 ± 0.38
−2.82 ± 0.66
Head kidney
3.18 ± 0.84
2.52 ± 0.54
2.73 ± 0.85
2.19 ± 0.64
2.00 ± 0.55
Liver
2.83 ± 1.53
−1.82 ± 0.59
1.00 ± 0.23
−1.90 ± 0.54
−2.00 ± 0.57
Thymus
1.06 ± 0.39
−1.22 ± 0.37
1.35 ± 0.55
1.02 ± 0.30
1.42 ± 0.43
tlr20
Spleen
−1.58 ± 0.29
−1.71 ± 0.38
−1.23 ± 0.21
1.08 ± 0.27
−1.02 ± 0.25
Head kidney
1.29 ± 0.20
−1.40 ± 0.21
1.07 ± 0.17
1.24 ± 0.18
1.49 ± 0.21
Liver
1.55 ± 0.23
1.74 ± 0.20
2.01 ± 0.19
2.20 ± 0.25
2.02 ± 0.26
Thymus
−1.17 ± 0.24
1.32 ± 0.35
−1.22 ± 0.32
1.19 ± 0.26
1.42 ± 0.31
tlr21
Spleen
−1.39 ± 0.28
−3.82 ± 0.46
−1.83 ± 0.40
−1.25 ± 0.35
−1.03 ± 0.19
Head kidney
−1.06 ± 0.26
−2.96 ± 0.61
−3.36 ± 0.51
−1.65 ± 0.26
−1.52 ± 0.24
Liver
1.67 ± 0.48
1.61 ± 0.44
1.34 ± 0.21
2.16 ± 0.58
1.51 ± 0.34
Thymus
1.07 ± 0.18
−1.31 ± 0.26
−1.47 ± 0.29
1.00 ± 0.21
−1.13 ± 0.21
tlr22a1
Spleen
1.21 ± 0.27
−1.18 ± 0.22
−1.10 ± 0.23
1.01 ± 0.27
−1.99 ± 0.45
Head kidney
1.55 ± 0.50
1.41 ± 0.34
1.51 ± 0.56
1.06 ± 0.24
−1.75 ± 0.44
Liver
2.12 ± 0.52
1.94 ± 0.43
2.50 ± 0.58
3.23 ± 1.00
1.41 ± 0.34
Thymus
1.24 ± 0.21
−1.11 ± 0.29
1.11 ± 0.35
1.07 ± 0.23
−1.46 ± 0.23
tlr22a2
Spleen
3.02 ± 0.64
1.21 ± 0.24
1.11 ± 0.11
1.89 ± 0.39
1.53 ± 0.30
Head kidney
6.14 ± 2.37
3.81 ± 0.70
1.63 ± 0.32
3.27 ± 0.70
2.71 ± 0.48
Liver
3.51 ± 0.79
4.41 ± 0.97
2.94 ± 0.85
3.85 ± 0.97
3.12 ± 0.80
Thymus
1.37 ± 0.25
1.83 ± 0.33
1.48 ± 0.40
1.23 ± 0.34
1.15 ± 0.28
nod1
Spleen
−1.05 ± 0.15
−1.60 ± 0.20
−1.01 ± 0.14
1.07 ± 0.12
−1.13 ± 0.13
Head kidney
−1.22 ± 0.23
−3.08 ± 0.61
−1.60 ± 0.26
−1.31 ± 0.32
−1.59 ± 0.35
Liver
1.20 ± 0.18
1.26 ± 0.19
1.37 ± 0.19
1.36 ± 0.30
1.74 ± 0.34
Thymus
−1.02 ± 0.21
1.45 ± 0.33
1.35 ± 0.39
−1.04 ± 0.27
−1.15 ± 0.26
Significant expression difference () between the infected and the control groups is indicated in bold.
(b)
Gene symbol
Tissues
Fold change values of infected group relative to the control
6 h
12 h
24 h
48 h
72 h
myd88
Spleen
1.44 ± 0.25
1.68 ± 0.24
1.33 ± 0.33
1.36 ± 0.22
1.25 ± 0.20
Head kidney
1.37 ± 0.16
1.33 ± 0.20
1.22 ± 0.22
1.45 ± 0.29
1.04 ± 0.12
Liver
2.75 ± 0.48
6.68 ± 0.63
3.34 ± 0.76
4.37 ± 1.48
6.78 ± 1.89
Thymus
1.08 ± 0.14
−1.13 ± 0.23
1.01 ± 0.21
1.09 ± 0.17
1.00 ± 0.14
irak4a
Spleen
−1.02 ± 0.14
−1.20 ± 0.10
1.26 ± 0.09
1.03 ± 0.16
−1.06 ± 0.14
Head kidney
1.04 ± 0.11
−1.25 ± 0.16
−1.36 ± 0.19
−1.31 ± 0.18
−1.39 ± 0.19
Liver
1.38 ± 0.14
1.67 ± 0.14
1.87 ± 0.24
1.51 ± 0.15
1.86 ± 0.23
Thymus
1.17 ± 0.16
1.10 ± 0.18
1.08 ± 0.18
1.26 ± 0.17
1.71 ± 0.23
irak1
Spleen
2.81 ± 0.43
2.86 ± 0.24
1.56 ± 0.17
1.51 ± 0.22
1.13 ± 0.20
Head kidney
2.24 ± 0.35
2.54 ± 0.47
1.23 ± 0.15
1.34 ± 0.17
1.55 ± 0.18
Liver
2.60 ± 0.50
3.93 ± 0.49
2.12 ± 0.35
2.14 ± 0.30
2.34 ± 0.34
Thymus
1.11 ± 0.13
−1.07 ± 0.19
−1.25 ± 0.21
−1.14 ± 0.16
1.13 ± 0.15
traf6
Spleen
1.10 ± 0.15
1.29 ± 0.13
1.26 ± 0.13
1.21 ± 0.16
1.15 ± 0.13
Head kidney
1.05 ± 0.09
1.49 ± 0.15
1.36 ± 0.14
1.57 ± 0.15
1.37 ± 0.15
Liver
1.27 ± 0.09
2.21 ± 0.27
2.21 ± 0.30
2.04 ± 0.33
2.17 ± 0.25
Thymus
1.18 ± 0.15
−1.04 ± 0.11
−1.01 ± 0.15
−1.03 ± 0.13
1.78 ± 0.22
tollip
Spleen
1.06 ± 0.19
1.21 ± 0.21
1.29 ± 0.22
1.15 ± 0.23
1.24 ± 0.21
Head kidney
1.31 ± 0.21
−1.05 ± 0.24
1.14 ± 0.12
1.27 ± 0.14
1.11 ± 0.10
Liver
1.62 ± 0.48
1.73 ± 0.54
2.74 ± 0.87
3.44 ± 1.14
4.53 ± 1.43
Thymus
1.03 ± 0.15
−1.11 ± 0.29
1.05 ± 0.24
−1.04 ± 0.19
1.29 ± 0.26
Significant expression difference () between the infected and the control groups is indicated in bold.