Increased Performances of the Biological Diagnosis of the Antiphospholipid Syndrome by the Use of a Multiplex Assay

Figure 1

Principle of the multiplex assay. Each of the 3 antigens (cardiolipin, B2GP1, and FII) is coated on specific microspheres defined by a unique spectral signature (discriminator signal). Patients’ serums are incubated with a calibrated mixture of the 3 microspheres. The autoantibodies binding is detected by a biotinylated anti-human IgG antibody and a PE labeled streptavidine (detection signal). Both signals are analyzed in a FIDIS flow cytometer.