Scaffolded Antigens in Yeast Cell Particle Vaccines Provide Protection against Systemic Polyoma Virus Infection

Figure 3

Sequence of the pGUL2-VP1-GFP expression cassette showing the duplicated upstream GAL1/10 UAS fragments (blue), the first (357 bp) in GAL1-GAL10 orientation and the second (353 bp) inverted. These are followed by the pGDH fragment (388 bp, also blue) completing the hybrid promoter. The reading frame for strain BG VP1 (1152 bp, red) is fused to GFPbex (915 bp, orange) and the two tPGK terminator segments (314 and 381 bp, blue) complete the cassette, as shown schematically in Figure 2.