The Role of Interleukin-23 in the Early Development of Emphysema in HIV1⁺ Smokers

Figure 4

Effect of IL-23 on MMP-9 expression in AM/lymphocyte coculture. (a) Effect of IL-23 (0.1–10 ng/mL) on AM/lymphocyte cocultures () relative MMP-9 gene expression (mean ± SEM, , ANOVA). (b) Gelatin zymography analysis of MMP-9 activity in conditioned media of HIV1⁻ healthy smoker AM/lymphocyte cocultures stimulated with IL-23. Shown is a representative gel. Lanes 1, 2: control; lanes 3, 4: IL-23, 0.1 ng/mL; lanes 5, 6: 1 ng/mL; lanes 7, 8: 10 ng/mL; lanes 9, 10: 20 ng/mL; lanes 11, 12: 40 ng/mL; and lanes 13, 14: 80 ng/mL. (c) Quantitation of gelatin zymography analysis of MMP-9 activity in conditioned media from 10 HIV1⁻ nonsmokers (), HIV1⁻ smokers (), and HIV1⁻ smokers with low DLCO () after stimulation with IL-23 (0–80 ng/mL; mean ± SEM, ANOVA).

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