The Role of Interleukin-23 in the Early Development of Emphysema in HIV1⁺ Smokers

Figure 5

Evaluation of AM and BAL lymphocytes for expression of surface IL-23R. (a) Flow cytometry analysis of cells recovered by lavage from HIV1⁻ smokers with low DLCO. Shown is a representative image from 3 similar experiments. Cells were stained with human IgG-PE (negative control) and mouse anti-human IL-23R-PE antibody 1 : 10. Ordinate: side scatter (SSC). Abscissa: forward scatter (FSC). In the first row, a gated population of AM does not show staining with IL-23R-PE. In the second row, a gated population of lymphocytes shows almost 50% of cells with positive IL-23R staining. (b) CD68 bead-purified AM do not respond to IL-23. Using detachable CD68-labeled magnetic beads, cells recovered by lavage were treated and AM were positively selected based on their surface expression of CD68. Isolated CD68+ cells were cultured and MMP-9 upregulation upon stimulation with IL-23 was compared to cultures of AM purified by adherence only. In contrast to AM purified via the adherence method, CD68+ AM () do not upregulate MMP-9 upon stimulation with IL-23 (mean ± SEM).

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