Prophylactic Chronic Zinc Administration Increases Neuroinflammation in a Hypoxia-Ischemia Model

Figure 7

Histopathological evaluation of hippocampus in rats treated with zinc. Paraffin-embedded tissue sections of 3 μm were stained with hematoxylin and eosin. Control, untreated rat; CZn15 d, rats with chronic administration of zinc; Zn15 d+CCAO 8 h, rats with chronic administration of zinc before CCAO and evaluations at 8 h after reperfusion; Zn15 d+CCAO 7 d, rats with chronic administration of zinc before CCAO and evaluations at 7 days after reperfusion. Dentate gyrus ((a), (d), (g), and (j)), CA1 ((b), (e), (h), and (k)), and CA3 ((c), (f), (i), and (l)) regions of the hippocampus. Apoptotic cells (dark arrow); necrosis (clear arrowhead). (((m), DG), ((n), CA1), and ((o), CA3)) Values of cell counts and color intensity (# pixels/μm²) using ImageJ, Java-based image processing program. when compared with the control group; unpaired Student’s -test; .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)

(m)

(n)

(o)