Research Article
A Fusion Protein Consisting of the Vaccine Adjuvant Monophosphoryl Lipid A and the Allergen Ovalbumin Boosts Allergen-Specific Th1, Th2, and Th17 Responses In Vitro
Figure 2
The MPLA : Ova fusion protein boosts mDC-derived cytokine secretion compared to the mixture of both components. Cytokine secretion determined from either BALB/c mDC (3.2 × 105 cells/mL, a) or BALB/c mDC (3.2 × 105 cells/mL) plus DO11.10 CD4+ T cell (8.0 × 105 cells/mL, >95% purity) cocultures (b) stimulated with equimolar amounts of Ova (white bars), MPLA (light grey bars), MPLA + Ova (dark grey bars), and the MPLA : Ova fusion protein (black bars) for 72 h and analyzed by ELISA. ELISAs were performed using either BD OptEIA™ ELISA (BD Biosciences) or Ready-SET-Go! ELISA Sets (eBiosciences). Data are mean results of two independent experiments ± SD. The hypothesis of a significant higher cytokine secretion among all three concentrations used for stimulation was tested with a two-factorial analysis of variance (ANOVA) with factors stimulus (0.2, 1.0, and 5.0) and group (“MPLA + OVA” or “MPLA : OVA”). For statistical significant results the following convention was used: value < 0.05, value < 0.01, and value < 0.001. The statistical analysis was performed with SAS®/STAT software, version 9.4, SAS System for Windows.
| (a) |
| (b) |