Differences between Mycobacterium-Host Cell Relationships in Latent Tuberculous Infection of Mice Ex Vivo and Mycobacterial Infection of Mouse Cells In Vitro

Figure 10

The phagocytic receptor CD35 on the cells in the fragments of mouse splenic granulomas. The scale bars are 10 μm each. ((b)-(c)) Nuclei stained by DAPI (blue signal). (a) Immunochemical localization of CD35 on granuloma cells and control mouse peritoneal macrophages (Mph). The brown color indicates the presence of the receptor in these cells. The black arrows indicate macrophages stained for this marker. (b) Confocal immunofluorescent localization of CD35 (red signal) on the cells in the cultures of bone marrow macrophages following infection with BCG in vitro and after culture for several hours. Macrophages stained for CD35 are indicated by the white arrows. (c) Confocal immunofluorescent localization of GM-CSF (green signal) and CD35 (red signal) in granuloma macrophages. In the lower-right panel, the same fragment as in the other panels restained for acid-fast BCG-mycobacteria by the ZN method. The black arrow on the ZN image indicates the macrophage region with BCG-mycobacteria reproducing in it. Abbreviations as in Figure 1.

(a)

(b)

(c)