Differences between Mycobacterium-Host Cell Relationships in Latent Tuberculous Infection of Mice Ex Vivo and Mycobacterial Infection of Mouse Cells In Vitro

Figure 13

Confocal immunofluorescent localization of vimentin (green signal) and CD11b (red signal) in the cells in a fragment of a mouse splenic granuloma. The scale bars are 10 μm each. Colocalization of the markers on the confocal images of cells (yellow signal). Nuclei are stained by DAPI (blue signal). In the right panel, the same fragment as in the other panels restained for acid-fast BCG-mycobacteria by the ZN method. Macrophages with BCG-mycobacteria reproducing in them are indicated by the white arrows on the fluorescent images and the black arrows on the ZN image. Abbreviations as in Figure 1.