Differences between Mycobacterium-Host Cell Relationships in Latent Tuberculous Infection of Mice Ex Vivo and Mycobacterial Infection of Mouse Cells In Vitro

Figure 14

The S100 proteins in the cells in the fragments of mouse splenic granulomas and control mouse peritoneal macrophages (Mph). The scale bars are 10 μm each. In the left panel, confocal immunofluorescent localization of S100 proteins (green signal) and CD11b (red signal). Colocalization of the markers on confocal images of cells (yellow signal). Nuclei are stained by DAPI (blue signal). In the central and right panels, immunochemical localization of S100 proteins in the cells. The brown color indicates the presence of the markers in these cells. The black and red arrows indicate macrophages and a dendritic cell, respectively, with increased production of S100 proteins. Abbreviations as in Figure 1.