Differences between Mycobacterium-Host Cell Relationships in Latent Tuberculous Infection of Mice Ex Vivo and Mycobacterial Infection of Mouse Cells In Vitro

Table 1

Proinflammatory cytokines IFN and IL-1 and growth factor GM-CSF in the macrophages of granulomas (Gran) isolated from the spleens of mice after 20 days (/20 d), one month (/1 m), and two months (/2 m) following BCG infection in vivo and after ex vivo culture, in peritoneal (Per-Mph) and bone marrow macrophages (BM-Mph) infected with BCG in vitro (BCG) or without infection (controls), in the peritoneal macrophages isolated from mice on day 20 after intraperitoneal BCG infection (Per-Mph-20 d), and after culture for several hours.

Cells	Cytokine-positive cells, %
	IFN		IL-1	GM-CSF
	Macrophages	Lymphocytes	Macrophages	Macrophages
Per-Mph-control-24 h	0.51 ± 0.41	0	2.91 ± 1.45	3.75 ± 0.56
Per-Mph-BCG-4 h	0.28 ± 0.2	0	7.07 ± 2.28	ND
Per-Mph-BCG-24 h	0.36 ± 0.23	0	3.06 ± 2.1	ND
BM-Mph-control-48 h	0.37 ± 0.21	0	5.66 ± 1.39	2.54 ± 1.59
BM-Mph-BCG-4 h	0.34 ± 0.25	0	1.16 ± 0.89	4.29 ± 1.48
BM-Mph-BCG-24 h	0.4 ± 0.26	0	3.39 ± 1.81	4.58 ± 3.34
BM-Mph-BCG-48 h	3.88 ± 1.68	0	8.39 ± 1.54	9.0 ± 1.75
Per-Mph-20 d (, 72 h)	11.32 ± 3.31	0	8.11 ± 1.53	3.68 ± 1.16
Gran/20 d (, 72 h)	41.85 ± 2.0	0	96.5 ± 1.23	26.54 ± 1.23
Gran/1 m (, 72 h)	17.69 ± 3.15	77.3 ± 4.68	17.39 ± 1.41	13.78 ± 1.01
Gran/2 m (, 48–96 h)	38.87 ± 1.89	21.59 ± 1.91	23.23 ± 1.03	25.28 ± 0.92
Gran 14/2 m (, 120 h)	7.41 ± 0.91	1.54 ± 0.92	ND	ND

Data are presented as the mean percentage of the number of cytokine-positive cells out of the macrophage population in the granulomas from different mice or in the cultures of mouse peritoneal and bone marrow macrophages, and the standard error of the mean. In each experiment, more than 1000 cells were analyzed at each time point. (comparisons of cytokine-positive macrophages in each Gran group and BM-Mph-BCG-48 h, BM-Mph-control-48 h); ND, not done (no staining was performed).