Monocyte Differentiation towards Protumor Activity Does Not Correlate with M1 or M2 Phenotypes

Figure 2

Morphological characterization of M1- or M2-polarized monocytes. Morphology observed for THP-1, U937, and primary monocytes under the indicated treatments. Images were taken with Motic Microscope at 200x magnification; scale bar indicates 50 μm. The most relevant features of differentiation were adherence, stronger in M1-polarizing treatments, and several cell shape changes in most cases presenting mixed shapes (round and spindle-like and with several membrane projections) and cell sizes.