Monocyte Differentiation towards Protumor Activity Does Not Correlate with M1 or M2 Phenotypes

Figure 6

M1 and M2 polarization induced by breast cancer cell lines. After treatment of all three types of monocytes with either MCF-7 or MDA-MB-231 conditioned media, cells were harvested and the panel of M1/M2-related markers was analyzed by flow cytometry. In (a) the result for M2-related marker CD36 in primary monocytes and U937 cells is depicted: dotted line histograms represent autofluorescence control and straight line and shaded histograms correspond to stimulation with MCF-7 and MDA-MB-231 conditioned media, respectively. In (b) the autofluorescence was used to normalize marker expression, a value of 1 to the level of autofluorescence, and expression after treatment with conditioned media was normalized dividing by this basal value. Fold changes are depicted and the markers represented are only those with changes ≥ 1.5-fold. White bars represent M1 markers and black bars M2 markers.

(a)

(b)