Immune Response Induced by an Immunodominant 60 kDa Glycoprotein of the Cell Wall of Sporothrix schenckii in Two Mice Strains with Experimental Sporotrichosis

Figure 1

Cell wall proteins of yeast-like cells of S. schenckii were separated on 2D-PAGE gels and detected with Coomassie Blue (a) and Western blot using polyclonal anti-gp60 primary antibodies and peroxidase-conjugated secondary antibodies (b). Signal was revealed with a DAB-peroxidase substrate solution. Dotted circles represent gp60 antigen (pH 4.5–5.5) showing several isoforms. The position of molecular weight standards is indicated on the left.

(a)

(b)