Immune Response Induced by an Immunodominant 60 kDa Glycoprotein of the Cell Wall of Sporothrix schenckii in Two Mice Strains with Experimental Sporotrichosis

Figure 4

Presence of anti-gp60 antibodies in mice prior to infection. Cell wall proteins of yeast-like cells of S. schenckii were analyzed by Western blot using sera from nonimmunized and gp60-preimmunized mice and peroxidase-conjugated secondary antibodies. Signal was revealed with a DAB-peroxidase substrate solution. Positive control (lane 1), nonimmunized C57BL/6 (lane 2), gp60-preimmunized C57BL/6 (lanes 3 and 4), nonimmunized BALB/c (lane 5), and gp60-preimmunized BALB/c (lanes 6 and 7) mice.